ABSTRACT: Antioxidant enzyme activity of groundnut leaves planted in crude oil contaminated soil was evaluated. 1.5 kg of soil each was placed in black polythene bags. They were divided into four groups (A-D) of five bags each. Each group was treated with different concentrations of crude oil (0 ml, 5 ml, 10 ml and 15 ml per 1.5 kg soil respectively). five seeds of Arachis hypogaea were planted in each bag. The seeds were cultivated for four weeks. The leaves sample was collected at weeks 2, week 3 and week 4. The Activity of catalase, superoxide dismutase and glutathione peroxidase were assayed. Also, lipid peroxidation index was determined. Our findings show that the activity of superoxide dismutase significantly
(p < 0.05) increases (2.0 × 10-6 to 2 × 10-6 u/mg) at all weeks. Catalase and lipid peroxidation reduced significantly (25 × 10-3 u/mg to 2 × 10-3 u/mg and 3.4 × 10-7 u/mg to 2.1 × 10-7 u/mg) at week 2 but in week 3 catalase activity increased (14 × 10-3 u/mg) but lipid peroxidation was not significant. At week 4 lipid peroxidation was significantly reduced (1.5 × 10-7 u/mg) but catalase was not significant. Glutathione peroxidase activity was significantly reduced (5.5 × 10-3 u/mg to 2.5 × 10-3 u/mg) at week 2 in contrast to weeks 3 and 4 which shows no significant difference. It was also observed that crude oil reduced the growth rate and chlorophyll content significantly. These data indicate that levels of crude oil in soil, affects negatively the oxidative and growth parameters of Arachis hypogaea.
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