Callogenesis

The study was aimed at generating callus from immature inflorescence and young leaf explants of coconut (Cocos nucifera L). Eeuwen’s medium which was supplemented with Murashige and Skoog macro salts, 6- Benzylamino purine (BAP) and 6-y dimethylally amino purine (2iP) at concentration of 1 mg/l each, was used as the culture medium. The effect of different concentrations of growth regulators when added to the culture medium was tested on callus generation.

The use of plant growth regulators in tissue culture media is critical for callogenesis. The type and concentration of auxin and cytokinin used have profound effect on callus induction. In this study, three types of auxin and one cytokinin were investigated for media supplementation. The auxins were naphthalene acetic acid (NAA), indole acetic acid (IAA) and 2,4-dichlorophenoxyl acetic acid (2,4-D), each at concentrations of 0, 2.5, 5.0, 7.5 and 10 mg/l. The cytokinin used was benzyl amino purine (BAP) at concentrations of 0, 0.1, 1.0 and 10.0mg/l.