Gel Electrophoresis

Optimization and Assessment of Different DNA Extraction Protocols in Cowpea (Vigna unguiculata L. Walp.)

The first and the most essential step in any genetic analysis is the extraction of good quality DNA. In this study, comparison of five different protocols of isolating total genomic DNA from Cowpea plant (Vigna unguiculata) was carried out. A total of 10 varieties of cowpea (V. unguiculata) were collected from IITA and planted in pots in a screenhouse in the Department of Botany, University of Ibadan, Nigeria.