Chicken.

Chronic toxicity study on neem, (Azadirachta indica) leaf aqueous extract in chicken (Gallus gallus domesticus)

This study on chronic toxicity of neem (Azadirachta indica) leaf aqueous extract in chicken (Gallus gallus domesticus) involved daily intraperitoneal administration of graded doses: 500, 1000, and 2000 mg/kg of the extract for a period of 18 days. Clinical symptoms of ruffled feathers, weakness, anorexia, depression, and dyspnoea were observed at 20 minutes post exposure extract-dose dependent, and death intervened on day 6 post exposure.