Male reproductive processes are impacted by edible fats. The present work studied the effect of the consumption of a sunflower-enriched diet on sperm variables and reproductive hormones in male rats. A total of twenty male Wistar rats were used in this study. The Control group received the regular rat chow while the Treated group received the regular rat chow supplemented with 25% linoleic sunflower oil. After 28 days sperm variables, and reproductive hormones were assessed. There was no significant difference in the serum testosterone and prolactin levels in the group fed sunflower-enriched diet compared to the control group. The serum LDH and the seminal vesicle fructose levels were significantly increased in the animals fed sunflower enriched diet compared to the control animals. The sperm count and sperm motility levels were significantly higher in the rats fed sunflower enriched diet compared to the control rats. The current findings imply that while 25% linoleic sunflower oil might increase sperm characteristics, it might not have the same effect on testosterone levels.