Polygalacturonase

Production, Purification and Comparison of Polygalacturonase from Aspergillus flavus, Aspergillus niger and Penicillium expansum

The research investigated the production, purification and comparison of polygalacturonase (PG) from cassava peels degrading fungi (Aspergillus flavus, Aspergillus niger and Penicillium expansum) isolated from biodegrading cassava peels. The fungi were isolated using cassava peel agar medium and the PG was produced using the solid state fermentation process. The PG was assayed using the modified dinitrosalicyclic acid reagent method and purified by using Sephadex G-100 and Sephadex C-50.