Wistar rats

The study was conducted for eight weeks to investigate the effects of 250mg/kg body weight of ethanolic leaf extract of Phyllanthus amarus on the morphology and reproductive functions of the ovary. Twenty-eight (28) adult female Wistar rats were categorized into four groups of seven rats each (n=7) with A (control), B (to assess implantation and resorption), C (to assess the litters), and D (to assess the effects on ovarian features and functions. Male adult rats of proven virility were mated with the female rats of groups B and C.

The study was to determine the level of toxicity of repeated administration of ethanol extract of a medicinal mushroom, Daldinia concentrica in animal model. Wistar albino rats of both sexes were sorted randomly into four groups of five rats each. Graded doses of 200, 400 and 800 mg/kg of the extract were administered orally, once daily for eight consecutive days to the rats in the respective groups, while control group received 10% tween 80 in distilled water only.