Chikwendu, C.I.

Characterization of Hospital Associated Methicillin Resistant Staphylococcus aureus (HA-MRSA) Using Random Amplified Polymorphic DNA (RAPD) Marker

Staphylococcus aureus is one of the most significant pathogens causing nosocomial infections. Twelve (12) methicilllin resistant Staphylococcus aureus (MRSA) strains obtained from clinical samples in Abia State, Nigeria was subjected to DNA fingerprinting by Random Amplified Polymorphic DNA (RAPD) analysis to examine their genotypic polymorphism and subsequently determine the degree of genetic relatedness among the various isolates. DNA extraction was carried out by boiling method and quantified using nanodrop1000 spectrophotometer.