crude oil

ABSTRACT: Antioxidant enzyme activity of groundnut leaves planted in crude oil contaminated soil was evaluated. 1.5 kg of soil each was placed in black polythene bags. They were divided into four groups (A-D) of five bags each. Each group was treated with different concentrations of crude oil (0 ml, 5 ml, 10 ml and 15 ml per 1.5 kg soil respectively). five seeds of Arachis hypogaea were planted in each bag.

A study was carried out to evaluate the changes in Superoxide dismutase (SOD) and Glutathione peroxidase activities (GPx) in Allium cepa roots exposed to crude oil. Crude oil used in this study was fractionated in water (ratio 1 to 2) to produce water-soluble fractions (WSF) and water insoluble fractions (WIF). Allium cepa was grown in soil treated with 2%, 5%, 10% or 20% of Whole Crude (WC), WIF or WSF for 14 days, while the control was with distilled water. Antioxidant enzyme activity was evaluated after treatment for 14days using standard protocols.

This experimental study was carried out to investigate the growth response, survival and morphology of Eichhornia crassipes (Mart.) Solms (Pontederiaceae) in crude oil polluted freshwater. The parameters measured include leaf diameter, stem girth, root length, chlorophyll content index and biomass production. The experimental treatment concentrations used were 0% (control), 1%, 2%, 3% and 4% (v/v). Using three replicates per treatment, the plants were exposed to all concentrations for eighteen days.

Phylogenetic and functional gene analysis of sulphate-reducing bacteria (SRB) from crude oil and produced water was carried out using molecular methods. The concentration of hydrogen sulphide (H2S) produced by SRB, as an indication of SRB presence, was determined using spectrophotometric sulphide assay. More H2S was observed to be produced at 55 ˚C than at 30 ˚C, with sample B found to produce more H2S at these temperatures than sample A. This implies more SRB contamination in sample B, possibly from subterranean water.