The efficacy of methanol leaf extract of Jatropha curcas was investigated through in-vitro assay against Trypanosoma brucei-brucei in 96 well micro-titer plates. Maceration method was used to obtain the leaf extract using absolute methanol. The extract was screened for phytochemicals and tested at various concentrations; 10 mg/ml, 5 mg/ml, 2.5 mg/ml, 1.25 mg/ml, 0.625 mg/ml, 0.3125 mg/ml, and 0.15625 mg/ml. The phytochemicals detected were alkaloids, saponins, flavonoids, tannins, resins, and terpenes. The result of the antiparasitic activity showed that methanol extract was effective against the T. b. bruicei parasite. Although, the extract trypanocidal activity is time and concentration dependent, the student t-test showed there were significant difference (p > 0.05) between the extract and drug associated with the trypanosomes mortalities after 6 hours incubation period. In conclusion, Jatropha curcas is likely to contain promising chemical compounds which can be utilized as an effective plant treatment against T. brucei - brucei parasite. Isolation of the effective secondary metabolites is recommended.