S. D. Yusufu

This study on chronic toxicity of neem (Azadirachta indica) leaf aqueous extract in chicken (Gallus gallus domesticus) involved daily intraperitoneal administration of graded doses: 500, 1000, and 2000 mg/kg of the extract for a period of 18 days. Clinical symptoms of ruffled feathers, weakness, anorexia, depression, and dyspnoea were observed at 20 minutes post exposure extract-dose dependent, and death intervened on day 6 post exposure.

Acute toxicity of Azadirachta indica (neem) leaf aqueous extract was tested on chickens; and the median lethal dose (LD50) was calculated. Both clinical signs, gross lesion and microscopic lesions at post mortem were recorded.