Vol 16, No 4 ,2015, African Scientist

Bacteriological Assessment of Ready-to-Eat Pawpaw (Carica papaya) Sold in Selected Locations in Benin City

This study was carried out to assess the microbiological quality of ready-to-eat papaya fruit from selected locations in Benin City. Standard bacteriological techniques using selective/differential media were employed for the isolation, purification and putative identification of selected bacterial isolates present in vended papaya fruits. The susceptibility and resistance of these bacterial isolates to common antibiotics was accessed. The public health implication of the identified isolates were also evaluated using their multiple antibiotic resistance (MAR) index.

Histological and Biochemical effects of Aqueous Extracts of Tectona grandis leaf on high-fat induced non-alcoholic fatty liver in albino rats

This study was done to determine the histological and biochemical effects of aqueous leaf extract of Tectona grandis on egg yolk induced non- alcoholic liver damage. The rats were randomly divided into four groups A, B, C, and D comprising of 8 rats each. Group A served as the control. Group B received egg yolk daily for 30 days, Group C was treated with 300 mg/kg body weight of aqueous extract of T. grandis alone while Group D was fed egg yolk and was treated with aqueous leaf extract of T. grandis for 30days.

Utilization of Banana Peel as a Substrate for Single Cell Protein and Amylase Production by Aspergillus niger

The suitability of banana peel medium for Aspergillus niger Single Cell Protein (SCP) and amylase production was studied. Single Cell Protein and amylase production was investigated using media designated as Supplemented Banana Peel (SBP), Glucose Banana Peel (GBP) and Unsupplemented Banana Peel (UBP). Biomass of A. niger protein content, amylase production and pH were determined using standard techniques in the course of submerged fermentation. Analyses of the various media showed that SBP medium produced the highest yield of A.

Microbiological Evaluation of Locally Processed Herbal Drugs Sold in Benin City

Plants that are referred to as “herbs” are not used as a food, but are grown and consumed as a garnishment for flavour enhancement, seasoning, and sometimes for alleged healing properties. This study investigates the microbiological quality of common herbal drugs sold in Benin City and antibacterial susceptibility profile of the Isolates. Herbal concoctions were purchased from various traditional medicine sales outlet in Benin City, Nigeria, coded as HA to HJ. Bacterial were cultured on Nutrients Agar while fungi were cultured on Potato Dextrose Agar.

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Effect of Ebola Virus on Bush Meat Sales in Benin City, Edo State, Nigeria

The Ebola virus and its link to bush meat species in Nigeria was received differently by people. While some believed and took precautions others did not and continued the consumption of bush meat. Consequently, a survey of ten markets in Benin City was undertaken between December 2014 and April 2015 to compare bush meat patronage during the pre- and post -Ebola period. Markets visited included, Oba Market, New Benin Market, Oliha market, Uwa market, Santana market, Evbuotubu market, Oregbeni market, Uselu market, Ekiosa market and Ugbighokho market.

Production of Single Cell Protein with Three Agro-Shell Wastes Using Bacillus subtilis

In this study, Bacillus subtilis isolated from decaying pineapple fruit peel was identified by both phenotypic and molecular techniques and employed for the production of single cell protein from three agricultural wastes: melon, groundnut and walnut shells. Chemical analysis showed these agro-wastes to contain high amounts of carbohydrate which ranged from 61.26 - 65.12 %. Using submerged fermentation, maximum yield of cell biomass was produced from the groundnut shell followed by melon shell as substrate for fermentation.

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Molecular Characterization of Bacteria Associated with the Spontaneous Fermentation of Bambara Nut in “Ogirri”

Fermentation is one of the oldest methods of food preservation known to man. Oil seeds and legumes have been fermented to give condiments. Molecular characterization of the bacteria associated with the spontaneous fermentation of Bambara nut (Vigna subterranea) seeds into “Ogirri” was carried out. Vigna subterranea seeds were subjected to pre-treatments of soaking and boiling and spontaneously fermented in 3 setups for a period of 96 h at 28±2 0C to yield ogirri product.

Production, Purification and Comparison of Polygalacturonase from Aspergillus flavus, Aspergillus niger and Penicillium expansum

The research investigated the production, purification and comparison of polygalacturonase (PG) from cassava peels degrading fungi (Aspergillus flavus, Aspergillus niger and Penicillium expansum) isolated from biodegrading cassava peels. The fungi were isolated using cassava peel agar medium and the PG was produced using the solid state fermentation process. The PG was assayed using the modified dinitrosalicyclic acid reagent method and purified by using Sephadex G-100 and Sephadex C-50.

Contrast Sensitivity and Reaction Time in Cataract Patients: A Comparative Analysis of Pre and Post Cataract Surgery

The purpose of this study was to measure and compare pre and post-operative Contrast sensitivity (CS) and Reaction Times (RTs) in cataract patients. CS provides valuable information on visual function, independent of visual acuity (VA). It represents the ability of the eye to detect a slight difference in luminance of two regions without distinct contours. This visual function or ability is affected by various disease conditions of the eye including cataract.

Corticosteroid Induced Ocular Hypertension in Feline Model and Its Effects on White Blood Cell Count

Prolong topical or systemic administration of corticosteroid produces undesirable side effects like increase intraocular pressure, lens opacities, neutrophilia, eosinopenia. This study was aimed at finding out the relationship between ocular hypertension and differential white blood cell count using corticosteroid induced ocular hypertension in feline model. A total of 5 cats were used for this study. The five cats were grouped into two (control and experimental). The control consisted of one cat while the experimental group consisted of four cats.