Volume 17, No 1 , 2016

Chemical Analysis and Biological Activity of Natural Preservative from Beet root (Beta vulgaris) Against Foodborne Pathogens and Spoilage Organisms.

Proximate and elemental analysis of Beta vulgaris was carried out using standard procedures. Ethanolic and aqueous extract of the root were analysed qualitatively and quantitatively for phytochemicals. The agar well diffusion assay was employed to determine the antibacterial activity of both ethanolic and water extracts of Beta vulgaris. Carbohydrate, protein, fat/oils, ash and moisture content were 9.50±0.01, 1.58±0.00, 0.23±0.00,1.43±0.01 and 88.69±1.20 % respectively. The mineral content ranged from 1.90±0.01 to 15.98±0.01 % with 

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In vitro cytotoxic effect of aqueous and hydroethanolic leaf extracts of Stachytarpheta cayennensis on the human pancreatic cell line (ASPC-1)

There is an urgent need for agents with improved efficacy against pancreatic cancer, as only 20% of pancreatic cancer patients undergoing chemotherapy respond to treatment. This study investigated the antiproliferative attributes of hydroethanolic and aqueous leaf extracts of Stachytarpheta cayennensis on human pancreatic carcinoma cell lines (AsPC-1). Human pancreatic (AsPC-1) cells were seeded (1.07×105 cells/well) into 96 well microtitre plate, allowed to stabilize, then exposed for 24hrs to graded (0 – 10000 μg ml-1) 

Callogenesis of coconut (Cocos nucifera L.) using immature inflorescence and young leaf explants

The study was aimed at generating callus from immature inflorescence and young leaf explants of coconut (Cocos nucifera L). Eeuwen’s medium which was supplemented with Murashige and Skoog macro salts, 6-Benzylamino purine (BAP) and 6-y dimethylally amino purine (2iP) at concentration of 1 mg/l each, was used as the culture medium. The effect of different concentrations of growth regulators when added to the culture medium was tested on callus generation.

Alkaloid, Flavonoid, Fatty Acid and Vitamin Profiles of the Unripe Fruit of Solanum melongena L. (Round Variety)

 Solanum melongena L. (round variety) is an eggplant that is commonly called “garden egg” in Nigeria. The unripe fruit is often eaten as snack, dessert or vegetable. The dried fruit powder was evaluated for its alkaloid, flavonoid and fatty acid profiles by Gas Chromatography (GC). The vitamin profile was estimated by High Performance Liquid Chromatography (HPLC). The alkaloid profile showed the presence of high amounts of solamargine (134 ± 0.719 mg/100g) and solasonine (115 ± 0.455 mg/100g), with other alkaloids present in lower 

Characterization of antibiogram susceptibility profile of Vibrio species isolated from fresh vegetables

Fresh vegetables water leaf (Talinum traingnlare), pumpkin leaf (Telfairia occidentalis) and scent leaf (Ocinum gratissimum) collected from urban markets in Benin City, Nigeria, were investigated for the presence of Vibrio species using standard microbiological techniques. The antibiotic susceptibility profiles of the isolates against a panel of 13 antibiotics, was carried out according to the method described by The National Committee for Clinical Laboratory Standards (NCCCLS).

Antibiotic Resistance and Plasmid Profile of Bacterial Isolates from Pork Meat Retailed in Benin City

 The use of antibiotics in animal husbandry has helped in the reduction of animal diseases. However, health workers as well as enlightened consumers are becoming more concerned with the health implications such as resistance associated with the use of antibiotics since the same group of antibiotics are used in human medicine.Sixty (60) samples from pork meat, piggery environment and abbatoir effluent were screened for the presence of bacteria by selective and differential cultural procedures. Susceptibility of isolates to antibiotics and plasmid 

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Physiological Response of Human Epithelial Squamous Carcinoma (HEp-2) cells to a Di-Herbal Formulation

Senna alata and Senna podocarpa are medicinal plants widely used in complementary and alternative medicine in the treatment of various diseases, in southwestern Nigeria. The in vitro cytotoxic and genotoxic potential of a formulation composed of methanolic leaf extract of S. alata (75%) and S. podocarpa (25%), on Human Epithelial Squamous Carcinoma (HEp-2) cells was investigated.

Histopathological Evaluation of the Effects of Vitamin C on Cisplatin-induced testicular damage in Wistar albino Rats

 Cisplatin is a very effective chemotherapeutic agent used in treating various types of cancer, but its application may have damaging effects on testicular morphology. In this study, the effect of vitamin C, a known antioxidant, on cisplatin-induced testicular damage was investigated. Thirty Wistar rats were grouped into six groups (A-F) of five rats per group.

Physicochemical analysis of automobile waste oil on soil quality in Benin City, Nigeria

Sustainable use of soil on which agriculture depends is absolutely necessary for agricultural productivity. Soil samples were collected from two auto-mechanic workshops sited at Technical College Road (Station A) and Textile Mill Road (Station B) while the control was at EDPA Housing Estate, all in Benin City with the aid of a split soil core sampler at a depth of 0  10cm. The Physicochemical parameters of the samples were analysed using standard methods and revealed pH mean value of 7.27 at station A and 6.83 at station B respectively.

Heavy Metals Levels in Dried Fish Consumed in Benin and Warri Metropolis; Levels and Health Risk Assessment

Heavy metals levels in dried Clarias gariepinus and Channa obscura from three major markets in Benin metropolis were investigated in this work. Samples were purchased, dried and digested by standard method and the metals levels were determined using Atomic Absorption Spectrophotometer (AAS). The mean levels of cadmium and lead (0.48±0.06 and 1.36±1.13 mg/kg) and (0.62±0.45 and 5.60±7.43 mg/kg) for Clarias gariepinus and Channa obscura were found to be higher than EU standard.