Vegetables are good sources of nutrients and can be contaminated by bacteria thereby causing spoilage and food-borne illnesses. The aim of this study was to determine the prevalence of Enterobacteriaceae in fresh vegetables sold in open markets in Benin City, Edo State. Thirty fresh vegetables samples comprising of scent, utazi, uziza, pumpkin and okazi leaves were obtained from three open markets. Isolation was carried out using pour plate technique and isolates were identified based on cultural, morphological and biochemical characteristics. The isolates were subjected to acid and bile salt tolerance test at pH 3.0 and 0.3 % bile salt concentration respectively. The Enterobacteriaceae count ranged from 0.02 ± 0.01 × 106 cfu/g (Uselu market) to 2.01 ± 0.04 × 106 cfu/g (New Benin market). The identified isolates were Proteus mirabilis, Salmonella spp., Escherichia coli, Enterobacter aerogenes (PUO), Citrobacter freundi, Klebsiella pneumoniae and Enterobacter aerogenes (UTN). The isolate E. aerogenes (PUO) had the highest occurrence of 22.94 % while P. mirabilis had the lowest occurrence of 6.42 %. Results of the acid tolerance test showed that Enterobacter aerogenes (UTN) had the lowest percentage viability of 81.43% while Salmonella spp. had the highest percentage viability of 100% after 3 h of incubation. Salmonella spp. had the lowest percentage viability of 87.80% while Klebsiella pneumoniae, Escherichia coli, Enterobacter aerogenes (PUO) and Citrobacter freundii had the highest percentage viabilities of 100% for bile salt tolerance, after 3 h of incubation. The high bacterial counts in this study could be attributed to poor hygienic practices at the markets. Therefore, proper public education on good food handling practices in markets is advised.