Minimum inhibitory concentration

The increase in antibacterial resistance has led to the urgent need for novel therapeutic agents, and recently, plant-based antibacterials have emerged as a promising substitute. This study aimed to investigate the antibacterials activity of the aqueous and ethanol extracts of the Carica papaya seeds against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, and Staphylococcus aureus. C. papaya seeds were collected, dried, and ground into powder, followed by the extraction of their bioactive compounds using water and ethanol solvents.

This research determines the antibacterial activity of the aqueous and methanol root extracts of male and female Carica papaya and Garcinia kola individually and in biherbal formulations using the agar diffusion method. The dried roots of the two plants were powdered separately. Garcinia kola (100 g) and Carica papaya (100 g) were macerated separately in boiled water and absolute methanol, Garcinia kola (50 g) and Carica papaya (50 g) were combined (100 g) and macerated in boiled water and absolute methanol at room temperature (30°C ± 2°C) for 72 hours.