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Development of Congenic Mutants and Genetic Analysis of Virulence in Cryptococcus Neoformans

Cryptococcus neoformans strains were isolated from clinical source taken from the University of Benin Teaching Hospital (UBTH) and environmental source taken from New Benin Market. Two strains were selected; clinical isolate 4832 and environmental isolate NBM5. They were determined to be of two different mating types, due to differences in their susceptibility to the antifungal agent fluconazole. In order to check the involvement of mating type locus in the virulence of C. neoformans, congenic mutants were developed.

Bioconcentration and Translocation Factors of Heavy Metals in Rhizophora racemosa and Sediments from Egbokodo Mangrove Swamp, Delta State, Nigeria

The mangrove ecosystem is well known to be impacted by anthropogenic activities such as oil spillage, agricultural run-off, industrial effluents, and mining in coastal areas. These activities increase heavy metals accumulated by mangroves, which are eventually released back into the environment via decomposition. This study aimed to analyze the concentration of heavy metals including cadmium (Cd), Zinc (Zn), lead (Pb), and copper (Cu) in the roots and leaves of Rhizophora racemosa and mangrove sediments obtained from Egbokodo mangrove swamp in Delta State, Nigeria.

Detection of icaD and MecA Genes and Antibiogram Profiling of Ear Swab Borne Staphylococcus aureus Isolates

Bacteriological analysis of fifty (50) ear swabs, collected from fifty (50) consenting male undergraduate students was conducted using routine procedures which included pour plating. Thirty one (31) Staphylococcus aureus were tentatively identified and subjected to biofilm production test and antibiotic susceptibility assay using Congo red agar and disc diffusion procedure. The presence of icaD and MecA genes students in the respective bacterial strains was ascertained using polymerase chain reaction and agarose gel electrophoresis.

Antimicrobial Activity of Mitracarpus scaber Leaf Extract against Some Human Pathogenic Microorganisms

The study was carried out with the aim to determine the antimicrobial effect of ethanolic leaf extract of Mitracarpus scaber against some clinical isolates. The leaves were screened for some phytocompounds using standard analytical methods.The antimicrobial activity of the leaf extract against Escherichia coli, Staphylococcus aureus and Candida albicans was carried out using agar well diffusion and broth dilution methods. The results of phytochemical studies revealed the presence of alkaloids, flavonoids, cardiac glycosides, triterpenes, phytosterols, tannins, and saponins.

Phytotoxicity Study on the Effects of Waste Engine Oil on the Anatomy of Sataria barbata (LAM.) KUNTH and Brachiaria deflexia (SCHUMMACH.) C.E. HUBB. EX ROBYNS

The present study explored the use of anatomical characters of Sataria barbata and Brachiaria deflexia as indicators of waste engine oil pollution. Four (4) kg of air dried soil was measured into perforated plastic buckets and treated with waste engine oil to obtain different concentrations [0% (control), 2%, 4%, 7% and 10%] on volume to weight basis. The experiment was set up in 3 replicates. The soil samples were allowed to stand for seven days before seedlings were planted. The plants were harvested after 8 weeks of transplanting.

Screening for Biodegradative Activity of Diesel Oil by Microorganisms Isolated from Petroleum Polluted Soil of a Mechanic Workshop at AKAD Community, Akure, Nigeria

This study was carried out to determine the bacterial and fungal flora of soils contaminated with used automobile engine oil at a mechanic workshop at AKAD community, Akure, Ondo State, Nigeria, with the aim of testing their ability to degrade diesel oil. Isolation and identification of microbes were done using standard microbiological techniques. Oil degrading activity of the isolates were assayed for using Bushnell Haas broth (BHB) supplemented with 1% diesel oil.