This study determined the optimum protein and digestible energy levels for Clarias gariepinus fingerlings fed local
The study was carried out with the aim to determine the antimicrobial effect of ethanolic leaf extract of Mitracarpus scaber against some clinical isolates. The leaves were screened for some phytocompounds using standard analytical methods.The antimicrobial activity of the leaf extract against Escherichia coli, Staphylococcus aureus and Candida albicans was carried out using agar well diffusion and broth dilution methods. The results of phytochemical studies revealed the presence of alkaloids, flavonoids, cardiac glycosides, triterpenes, phytosterols, tannins, and saponins.
The present study explored the use of anatomical characters of Sataria barbata and Brachiaria deflexia as indicators of waste engine oil pollution. Four (4) kg of air dried soil was measured into perforated plastic buckets and treated with waste engine oil to obtain different concentrations [0% (control), 2%, 4%, 7% and 10%] on volume to weight basis.The experiment was set up in 3 replicates. The soil samples were allowed to stand for seven days before seedlings were planted. The plants were harvested after 8 weeks of transplanting.
This study was carried out to determine the bacterial and fungal flora of soils contaminated with used automobile engine oil at a mechanic workshop at AKAD community, Akure, Ondo State, Nigeria, with the aim of testing their ability to degrade diesel oil. Isolation and identification of microbes were done using standard microbiological techniques. Oil degrading activity of the isolates were assayed for using Bushnell Haas broth (BHB) supplemented with 1% diesel oil.
Genome editing is used to make targeted modifications to the genome of eukaryotic cells. There are many potential applications of genome editing in human pluripotent stem cells (hPSCs) including the generation of knockout and reporter cell lines. This protocol describes a system for efficient genome editing in hPSCs using engineered transcription activator-like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeat (CRISPR) technology.
Prolonged culture of pluripotent and multipotent stem cells exposes the cells to strong selection pressures, often resulting in genomic alterations. Any genetic manipulation of the cells may further jeopardize their genomic stability. Genomic aberrations affect the differentiation capacity of stem cells, their stem cell identity and their tumorigenicity, and should thus be routinely evaluated for their proper use in basic research and in clinical trials.
The directed differentiation of human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) into hepatocytes could facilitate a rational study of the molecular mechanisms underlying human liver development as well as provide a renewable source of exogenous hepatocytes for drug toxicity testing and cell-based therapeutics. Moreover, if hepatocytes were produced from hiPSCs originating from patients with inborn errors of hepatic metabolism, such cells could be used for modeling liver disease.
The pluripotency of embryonic stem (ES) cells is the result of a highly dynamic equilibrium that is controlled by a complex network of transcription factors that confer unique transcriptional properties to ES cells. Regulation of gene expression appears to correlate with the presence of dual chromatin marks called bivalent domains at the promoters of poised developmental genes. These marks keep differentiation genes silenced but poised and ready to be activated or permanently repressed during differentiation.
‘Burukutu’ which is an indigenous alcoholic drink has been shown to be rich in bacterial diversity, with a total of thirteen (11) bacterial species isolated from the three stages of production (namely: steeping, mashing and maturation) sampled from two different selling points in Ilorin, metropolis. The bacterial species isolated are Streptococcus sp; Lactobacillus sp; Bacillus sp; Pseudomonas sp; Klebsiella sp; Staphylococcus sp; Enterococcus sp; Escherichia coli; Proteus sp; Leuconostoc sp and Micrococcus sp.
The piscicidal potential of water-extract mesocarp of Azardirachta indica was investigated in static biossay experiment with continuous aeration to determine its acute toxicity. The 96-hLC50 was 97.42mg/l while the threshold value was 31.12mg/l. The extract led to an initial increase in opercula ventilation rates which then decreased significantly. The computed regression equation (y) = 4.624 + 6.352 Logc. (r=0.72) and the fish exhibited respiratory distress, loss of balance and erratic swimming prior to mortality. The usefulness of A. indica as piscicidal plant is discussed.
