2

Staphylococcus aureus is one of the most significant pathogens causing nosocomial infections. Twelve (12) methicilllin resistant Staphylococcus aureus (MRSA) strains obtained from clinical samples in Abia State, Nigeria was subjected to DNA fingerprinting by Random Amplified Polymorphic DNA (RAPD) analysis to examine their genotypic polymorphism and subsequently determine the degree of genetic relatedness among the various isolates. DNA extraction was carried out by boiling method and quantified using nanodrop1000 spectrophotometer.

Aspergillus niger popularly known as industrial workhorse due to its fitness for industrial fermentation, has the potentials to secret citric acid. In view of surges in demand and growing markets for citric acid, the major attempt in this work was to determine how the product titres can be maximized. A. niger strains were isolated from various food substances. These strains were exposed to UV radiation at 356nm followed by subsequent subjection of the mutant strains to a medium containing chlorate as mutagen.

Microbial activities contribute significantly to the global economic burden of corrosion. The sulphatereducing bacteria (SRB) are major cause of biocorrosion in anoxic environments. This study investigated the corrosion inhibition efficiency of Gmelina arborea extracts against corrosion of carbon steel caused by SRB isolated from injection and produced water from Bonga oilfield. The SRB isolation, antimicrobial assay and corrosion experiments were carried out in a Coy anaerobic chamber.

Insects of the taxonomic order Coleoptera are recognized for considerable cellulolytic activity in their digestive tract. However, the cellulolytic activity of the gut microbiome of the African palm weevil (Rhynchoporus phoenicis), has not been reported. Thus, this study aimed at isolating cellulose degrading bacteria from the gut of the insect’s larva and assessingtheir cellulolytic activities. The larva of the insect was dissected and inoculated in carboxymethylcellulose-Congo red (CMC-CR) agar medium and incubated at room temperature (28±2) for 14 days.

This study assessed the air pollution tolerance index (APTI) of four plants around the Seplat Gas Flow Station in Sapele, Delta State. Fresh leaves of the plants were collected, weighed, dried and extracts were made. Standardized procedures for determining APTI were adopted. The physio-biochemical characteristics of the four plant species ranged from; pH 4.57–5.36, chlorophyll content 0.17-0.45 mg/g, RWC 41.0%–80.0%, ascorbic acid: 0.91 – 1.58 mg/g, while APTI varied from 5.75 – 6.99.

Numerous health benefits of consuming eggplant fruits (Solanum melongena L.) have been reported. This research was carried out to determine some of the constituents responsible for its nutritional and medicinal properties. The unripe fruits of Solanum melongena L. (round variety) were evaluated for its phytosterol, phenolic acid, amino acid and sugar profiles by gas chromatography. The Phytosterol analysis showed the presence of seven phytosterols and sitosterol (233 ± 0.852 mg/100g) was found to be significantly higher (p<0.01) than the other phytosterols.

 Below-ground carbon dioxide (CO2) emissions occur naturally at CO2 springs, but the risk of occurrence at other sites will increase as geologic CO2 storage is implemented to help mitigate climate change. This investigation examines the effect of controlled injection of below ground CO2 emission on wheat plant and soil microbial respiration where spring wheat (triticum aestivum L.) was grown. The study involved setting up eight (8) experimental plots (each 2.5 x 2.5m) for the growth of spring wheat.

Proximate and elemental analysis of Beta vulgaris was carried out using standard procedures. Ethanolic and aqueous extract of the root were analysed qualitatively and quantitatively for phytochemicals. The agar well diffusion assay was employed to determine the antibacterial activity of both ethanolic and water extracts of Beta vulgaris. Carbohydrate, protein, fat/oils, ash and moisture content were 9.50±0.01, 1.58±0.00, 0.23±0.00,1.43±0.01 and 88.69±1.20 % respectively. The mineral content ranged from 1.90±0.01 to 15.98±0.01 % with 

There is an urgent need for agents with improved efficacy against pancreatic cancer, as only 20% of pancreatic cancer patients undergoing chemotherapy respond to treatment. This study investigated the antiproliferative attributes of hydroethanolic and aqueous leaf extracts of Stachytarpheta cayennensis on human pancreatic carcinoma cell lines (AsPC-1). Human pancreatic (AsPC-1) cells were seeded (1.07×105 cells/well) into 96 well microtitre plate, allowed to stabilize, then exposed for 24hrs to graded (0 – 10000 μg ml-1) 

The study was aimed at generating callus from immature inflorescence and young leaf explants of coconut (Cocos nucifera L). Eeuwen’s medium which was supplemented with Murashige and Skoog macro salts, 6-Benzylamino purine (BAP) and 6-y dimethylally amino purine (2iP) at concentration of 1 mg/l each, was used as the culture medium. The effect of different concentrations of growth regulators when added to the culture medium was tested on callus generation.